Phosphodiesterase methods and protocols

• Other Authors: Lugnier, Claire (Editor)
• Format: Book
• Language: English
• Published: Totowa, N.J. Humana Press 2005
• Series: Methods in molecular biology v. 307
• Subjects: Phosphoric Diester Hydrolases > Laboratory Manuals > analysis; Phosphoric Diester Hydrolases > Laboratory Manuals > physiology; Biological Assay > Laboratory Manuals > methods; Protein Engineering > Laboratory Manuals > methods
• Online Access: Click Here to View Status and Holdings.

Adenosine 3 ,5 -cyclic monophosphate (cAMP) and guanosine 3 ,5 -cyclic monophosphate are ubiquitous nucleotides that have been described as the first and second messengers. In concert with intracellular calcium and IP3, they play a major role in the control of intracellular signaling, which orchestr...

Table of Contents:

• Study of cyclic adenosine monophosphate microdomains in cells / Marco Mongillo ... [et al.]
• High-resolution measurements of cyclic adenosine monophosphate signals in 3D microdomains / Jeffrey W. Karpen and Thomas C. Rich
• Cygnets : in vivo characterization of novel cGMP indicators and in vivo imaging of intracellular cGMP / Akira Honda ... [et al.]
• High-throughput screening of phosphodiesterase activity in living cells / Thomas C. Rich and Jeffrey W. Karpen
• Assessment of phosphodiesterase isozyme contribution in cell and tissue extracts / Thèrése Keravis, Rima Thaseldar-Roumié, and Claire Lugnier
• Localization of the cyclic guanosine 3', 5'-monophosphate-hydrolysing phosphodiesterase type 9 in rat brain by nonradioactive in situ hybridization / Wilma C.G. Van Staveren and Marjanne Markerink-Van Ittersum
• Determination of Ca2+/calmodulin-stimulated phosphodiesterase activity in intact cells / Chen Yan
• Adenovirus-mediated overexpression of murine cyclic nucleotide phosphodiesterase 3B / Faiyaz Ahmad ... [et al.]
• Identification of promoter elements in the 5'-flanking region of the murine cyclic nucleotide phosphodiesterase 3B gene / Hanguan Liu ... [et al.]
• Purification of PDE6 isozymes from mammalian retina / Dana C. Pentia ... [et al.]
• Cyclic guanosine 5'-monophosphate binding to the regulatory GAF domains of photoreceptor phosphodiesterase / Rick H. Cote
• Renaturation of the catalytic domain of PDE4A expressed in escherichia coli as inclusion bodies / Wito Richter, Thomas Hermsdorf, and Dietrich Dettmer
• Determining the subunit structure of phosphodiesterases using gel filtration and sucrose density gradient centrifugation / Wito Richter
• Crystallization of cyclic nucleotide phosphodiesterases / Hengming Ke, Qing Huai, and Robert X. Xu
• Generation of PDE4 knockout mice by gene targeting / S.-L. Catherine Jin, Anne M. Latour, and Marco Conti
• Immunoprecipitating PDE2 phosphorylated and inactivated by an associated protein kinase / J. Kelley Bentley
• Investigation of extracellular signal-regulated kinase 2 mitogen-actived protein kinase phosphorylation and regulation of activity of PDE4 cyclic adenosine monophosphate-specific phosphodiesterases / Elaine V. Hills, Miles D. Houslay, and George S. Baillie
• Radiolabeled ligand binding to the catalytic or allosteric sites of PDE5 and PDE11 / James L. Weeks ... [et al.]
• Analysis of dimerization determinants of the PDE6 catalytic subunits / Khakim G. Muradov, Kimberley K. Boyd, and Nikolai O. Artemyev
• Interaction between catalytic and inhibitory subunits of PDE6 / Nikolai O. Artemyev
• Purification, reconstitution on lipid vesciles and assays of PDE6 and its activator G protein, transducin
• Theodore G. Wensel, Feng He, and Justine A. Malinski.