Phosphodiesterase methods and protocols
Adenosine 3 ,5 -cyclic monophosphate (cAMP) and guanosine 3 ,5 -cyclic monophosphate are ubiquitous nucleotides that have been described as the first and second messengers. In concert with intracellular calcium and IP3, they play a major role in the control of intracellular signaling, which orchestr...
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| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J.
Humana Press
2005
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| Series: | Methods in molecular biology
v. 307 |
| Subjects: | |
| Online Access: | Click Here to View Status and Holdings. |
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| 245 | 0 | 0 | |a Phosphodiesterase methods and protocols |c edited by Claire Lugnier |
| 264 | # | 1 | |a Totowa, N.J. |b Humana Press |c 2005 |
| 264 | # | 4 | |c ©2005 |
| 300 | # | # | |a xii, 324 pages |b illustrations |c 23 cm |
| 336 | # | # | |a text |b txt |2 rdacontent |
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| 490 | 1 | # | |a Methods in molecular biology |v v. 307 |x 1064-3745 |
| 504 | # | # | |a Includes bibliographical references and index |
| 505 | 0 | # | |a Study of cyclic adenosine monophosphate microdomains in cells / Marco Mongillo ... [et al.] -- High-resolution measurements of cyclic adenosine monophosphate signals in 3D microdomains / Jeffrey W. Karpen and Thomas C. Rich -- Cygnets : in vivo characterization of novel cGMP indicators and in vivo imaging of intracellular cGMP / Akira Honda ... [et al.] -- High-throughput screening of phosphodiesterase activity in living cells / Thomas C. Rich and Jeffrey W. Karpen -- Assessment of phosphodiesterase isozyme contribution in cell and tissue extracts / Thèrése Keravis, Rima Thaseldar-Roumié, and Claire Lugnier -- Localization of the cyclic guanosine 3', 5'-monophosphate-hydrolysing phosphodiesterase type 9 in rat brain by nonradioactive in situ hybridization / Wilma C.G. Van Staveren and Marjanne Markerink-Van Ittersum -- Determination of Ca2+/calmodulin-stimulated phosphodiesterase activity in intact cells / Chen Yan -- Adenovirus-mediated overexpression of murine cyclic nucleotide phosphodiesterase 3B / Faiyaz Ahmad ... [et al.] -- Identification of promoter elements in the 5'-flanking region of the murine cyclic nucleotide phosphodiesterase 3B gene / Hanguan Liu ... [et al.] -- |
| 505 | 0 | # | |a Purification of PDE6 isozymes from mammalian retina / Dana C. Pentia ... [et al.] -- Cyclic guanosine 5'-monophosphate binding to the regulatory GAF domains of photoreceptor phosphodiesterase / Rick H. Cote -- Renaturation of the catalytic domain of PDE4A expressed in escherichia coli as inclusion bodies / Wito Richter, Thomas Hermsdorf, and Dietrich Dettmer -- Determining the subunit structure of phosphodiesterases using gel filtration and sucrose density gradient centrifugation / Wito Richter -- Crystallization of cyclic nucleotide phosphodiesterases / Hengming Ke, Qing Huai, and Robert X. Xu -- Generation of PDE4 knockout mice by gene targeting / S.-L. Catherine Jin, Anne M. Latour, and Marco Conti -- Immunoprecipitating PDE2 phosphorylated and inactivated by an associated protein kinase / J. Kelley Bentley -- Investigation of extracellular signal-regulated kinase 2 mitogen-actived protein kinase phosphorylation and regulation of activity of PDE4 cyclic adenosine monophosphate-specific phosphodiesterases / Elaine V. Hills, Miles D. Houslay, and George S. Baillie -- |
| 505 | 0 | # | |a Radiolabeled ligand binding to the catalytic or allosteric sites of PDE5 and PDE11 / James L. Weeks ... [et al.] -- Analysis of dimerization determinants of the PDE6 catalytic subunits / Khakim G. Muradov, Kimberley K. Boyd, and Nikolai O. Artemyev -- Interaction between catalytic and inhibitory subunits of PDE6 / Nikolai O. Artemyev -- Purification, reconstitution on lipid vesciles and assays of PDE6 and its activator G protein, transducin -- Theodore G. Wensel, Feng He, and Justine A. Malinski. |
| 520 | # | # | |a Adenosine 3 ,5 -cyclic monophosphate (cAMP) and guanosine 3 ,5 -cyclic monophosphate are ubiquitous nucleotides that have been described as the first and second messengers. In concert with intracellular calcium and IP3, they play a major role in the control of intracellular signaling, which orchestrates normal and pathophysiological responses. Downstream from the cyclic nucleotide synthesis by adenylyl and guanylyl cyclases, the multigenic family of cyclic nucleotide phosphodiesterases (PDEs), by specifically hydrolyzing cyclic nucleotides, controls cAMP and cGMP levels to maintain a basal state. Their critical role in intracellular sign- ing has recently designated them as new therapeutical targets. Several leading pharmaceutical companies are searching and developing new therapeutic agents that would potently and selectively inhibit PDE isozymes, notably PDE4 and PDE5. Nevertheless, the precise mechanism and the contribution of the various PDE isozymes in modulating intracellular signaling remain to be established. The aim of Phosphodiesterase Methods and Protocols is to provide a palette of a variety of conceptual and technical approaches designed to solve qu- tions concerning the role of PDEs, and ultimately of their different variants, in physiological functions as well as their implications in several pathologies |
| 650 | 1 | 2 | |a Phosphoric Diester Hydrolases |v Laboratory Manuals |x analysis |
| 650 | 1 | 2 | |a Phosphoric Diester Hydrolases |v Laboratory Manuals |x physiology |
| 650 | 2 | 2 | |a Biological Assay |v Laboratory Manuals |x methods |
| 650 | 2 | 2 | |a Protein Engineering |v Laboratory Manuals |x methods |
| 700 | 1 | # | |a Lugnier, Claire |e editor |
| 840 | # | # | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 307 |
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Cyclic nucleotide phosphodiesterases in health and disease