Cytochrome P450 protocols

• Other Authors: Phillips, Ian R. 1951- (Ian Randall) (Editor), Shephard, Elizabeth A. 1950- (Elizabeth Anne) (Editor)
• Format: Book
• Language: English
• Published: Totowa, N.J. Humana Press 2006
• Edition: Second Edition
• Series: Methods in molecular biology 320
• Subjects: Cytochrome P-450 Enzyme System > Laboratory manuals
• Online Access: Click Here to View Status and Holdings.

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450...

Table of Contents:

• Cytochrome P450 nomenclature, 2004 / David R. Nelson
• Spectral analyses of cytochromes P450 / John B. Schenkman and Ingela Jansson
• Establishment of functional human cytochrome P450 monooxygenase systems in Escherichia coli / Michael P. Pritchard, Lesley McLaughlin, and Thomas Friedberg
• Purification of cytochromes P450: products of bacterial recombinant expression systems / F. Peter Guengerich and Martha V. Martin
• Expression of recombinant flavin-containing monooxygenases in a baculovirus/insect cell system / Azara Janmohamed ... [et al.]
• Cytochrome P450 reconstitution systems / Hiroshi Yamazaki and Tsutomu Shimada
• Catalytic assays for human cytochrome P450: an introduction / Thomas K.H. Chang and David J. Waxman
• Enzymatic analysis of cDNA-expressed human CYP1A1, CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrate / Thomas K.H. Chang and David J. Waxman
• Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation / David J. Waxman and Thomas K.H. Chang
• Determination of CYP2B6 component of 7-ethoxy-4-trifluoromethylcoumarin O-deethylation activity in human liver microsomes / Thomas K.H. Chang, Charles L. Crespi, and David J. Waxman
• High-performance liquid chromatography analysis of CYP2C8-catalyzed paclitaxel 6[alpha]-hydroxylation / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman
• Determination of CYP2C0-catalyzed diclofenac 4'-hydroxylation by high-performance liquid chromatography / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman
• CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detection / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman
• CYP2D6-dependent bufuralol 1'-hydroxylation assayed by reverse-phase ion-pair high-performance liquid chromatography with fluorescence detection / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman
• Spectrophotometric analysis of human CYP2E1-catalyzed p-nitrophenol hydroxylation / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman
• Thin-layer chromotography analysis of human CYP23A-catalyzed testosterone 6[beta]-hydroxylation / David J. Waxman and Thomas K.H. Chang
• Determination of CYP4A11-catalyzed lauric acid 12-hydroxylation by high-performance liquid chromotography with radiometric detection / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman
• An isocratic high-performance liquid chromatography assay for CYP7A1-catalyzed cholesterol 7[alpha]-hydroxylation / David Waxman and Thomas K.H. Chang
• Use of 7-ethoxycoumarin to monitor multiple enzymes in the human CYP1, CYP2, and CYP3 families / David J. Waxman and Thomas K.H. Chang
• Benzydamine N-oxygenation as a measure of flavin-containing monooxygenase activity / Catherine K. Yeung and Allan E. Rettie
• Prochiral sulfoxidation as a probe for flavin-containing monooxygenases / Catherine K. Yeung and Allan E. Rettie
• Targeting antipeptide antibodies toward cytochrome P450 enzymes / Robert J. Edwards
• The Human Cytochrome P450 Allele Nomenclature Committee web site: submission criteria, procedures, and objectives / Sarah C. Sim and Magnus Ingelman-Sundberg
• Genotyping for cytochrome P450 polymorphisms / Ann K. Daly, Barry P. King, and Julian B.S. Leathart
• Hepatocyte cultures in drug metabolism and toxicological research and testing / Tamara VanHaecke and Vera Rogiers
• Isolation of rat hepatocytes / Peggy Papeleu ... [et al.]
• Rat hepatocyte cultures: conventional monolayer and cocultures with rat liver epithelial cells / Tom Henkens ... [et al.]
• Rat hepatocyte cultures: collagen gel sandwich and immobilization cultures / Mathieu Vinken ... [et al.]
• Hepatocytes in suspension / Greetje Elaut ... [et al.]
• Isolation of rat bone marrow stem cells / Sarah Snykers, Tamara Vanhaecke, and Vera Rogiers
• Transfection of primary cultures of rat hepatocytes / Mina Edwards
• Human hepatocyte culture / Lydiane Pichard ... [et al.]
• Determination of cellular localization of the expression of flavin-containing monooxygenase genes in mouse tissues by in situ hybridization / Azara Janmohamed, Ian R. Phillips, and Elizabeth A. Shephard
• Deletion of genes from the mouse genome using cre/loxP technology / Diana Hernandez ... [et al.]
• Characterization of targeted mouse embryonic stem cell chromosomes: karyotyping and fluorescence in situ hybridization of metaphase spreads / Diana Hernandez, Ian R. Phillips, and Elizabeth A. Shephard
• Microinjection of targeted embryonic stem cells and establishment of knockout mouse lines for fmo genes / Diana Hernandez ... [et al.]
• Use of reporter genes to measure xenobiotic-mediated activation of CYP gene transcription / Nick Plant.