Cytochrome P450 protocols

• Other Authors: Phillips, Ian R. 1951- (Ian Randall) (Editor), Shephard, Elizabeth A. 1950- (Elizabeth Anne) (Editor)
• Format: Book
• Language: English
• Published: Totowa, N.J. Humana Press 2006
• Edition: Second Edition
• Series: Methods in molecular biology 320
• Subjects: Cytochrome P-450 Enzyme System > Laboratory manuals
• Online Access: Click Here to View Status and Holdings.
• Physical Description: xiii, 363 pages illustrations 24 cm
• Bibliography: Includes bibliographical references and index
• ISBN: 9781588294418; 1588294412; 1592599982

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source