Human retrovirus protocols virology and molecular biology
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the inte...
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Other Authors: | |
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Format: | Book |
Language: | English |
Published: |
Totowa, New Jersey
Humana Press
2005
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Series: | Methods in molecular biology
v. 304 |
Subjects: | |
Online Access: | Click Here to View Status and Holdings. |
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041 | 0 | # | |a English |
090 | 0 | 0 | |a QR414.5 |b .H785 2005 |
245 | 0 | 0 | |a Human retrovirus protocols |b virology and molecular biology |c edited by Tuofu Zhu |
264 | # | 1 | |a Totowa, New Jersey |b Humana Press |c 2005 |
264 | # | 4 | |c ©2005 |
300 | # | # | |a xiv, 494 pages |b illustrations |c 24 cm |
336 | # | # | |a text |b txt |2 rdacontent |
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338 | # | # | |a volume |b nc |2 rdacarrier |
490 | 1 | # | |a Methods in molecular biology |v v. 304 |
504 | # | # | |a Includes bibliographical references and index |
520 | # | # | |a A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection |
650 | 1 | 2 | |a HIV |v Laboratory Manual |x isolation & purification |
650 | 2 | 2 | |a Retroviridae |v Laboratory Manual |x isolation & purification |
650 | 2 | 2 | |a Molecular Biology |v Laboratory Manual |x methods |
700 | 1 | # | |a Zhu, Tuofu |e editor |
840 | # | # | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 304 |
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