Human retrovirus protocols virology and molecular biology

A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the inte...

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Bibliographic Details
Other Authors: Zhu, Tuofu (Editor)
Format: Book
Language:English
Published: Totowa, New Jersey Humana Press 2005
Series:Methods in molecular biology v. 304
Subjects:
Online Access:Click Here to View Status and Holdings.
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520 # # |a A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection 
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650 2 2 |a Retroviridae  |v Laboratory Manual  |x isolation & purification 
650 2 2 |a Molecular Biology  |v Laboratory Manual  |x methods 
700 1 # |a Zhu, Tuofu  |e editor 
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