Resin microscopy and on-section immunocytochemistry

• Main Authors: Newman, G. R. 1943- Geoffrey R. (Author), Hobot, J. A. 1950- Jan A. (Author)
• Format: Book
• Language: English
• Published: Berlin New York Springer 2001
• Edition: Second Edition
• Series: Springer lab manuals
• Subjects: Immunohistochemistry > methods; Microscopy, Electron > methods; Plastic Embedding > methods; Tissue Preservation > methods; Acrylic Resins; Epoxy Resins
• Online Access: Click Here to View Status and Holdings.
• Physical Description: xix, 273 pages illustrations 25 cm
• Bibliography: Includes bibliographical references (p. 246-264) and index
• ISBN: 9783540672777; 354067277X

Since antibodies tagged with markers have been developed, immunocytochemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired